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Biblioteca(s): | INIA Las Brujas.
Tipo de la producción científica: | Artículos en Revistas Indexadas Internacionales
Circulación/Nivel: | B - 4
Fecha corriente: | 24/10/2014
Fecha de la última actualización: | 15/10/2019
Autoria: | ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M.
Afiliación: | DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay.
Título: | In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.
Año de publicación: | 2014
Fuente/Imprenta: | Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273.
ISSN: | 1948-5948
DOI: | 10.4172/1948-5948.1000155
Idioma: | Inglés

Contenido:

ABSTRACT.

Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability.

Thesagro: | ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS.
Categoría del tema: | F30 Genética vegetal y fitomejoramiento
URL: | http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (100217-1) - PXI - AP - DD - PP/JR.MICROB.BIOCHEM.TECHN./2014 -  -  - DIS